The Active Peptide from Shark Extra Handsets Liver (APSL) was expressed in E.coli BL21 cells.The cDNA encoding APSL protein was obtained from shark regenerated hepatic tissue by RT-PCR, then it was cloned in the pET-28a expression vector.The expressed fusion protein was purified by Ni-IDA affinity chromatography.SDS-PAGE and HPLC analysis showed th
ABSTRACT Prior to the advent of milk pasteurization and the use of defined-strain starter cultures, the production and ripening of cheese relied on the introduction and growth of adventitious microbes from the environment.This study characterized microbial community structures throughout a traditional farmstead cheese production continuum and evalu
Crickets (Order Orthoptera, Infraorder Gryllidea) are under-represented in New Zealand, with a total of eight species identified and formally described thus far.These include three endemic species in the family Trigonidiidae: the trig Trigonidium (Metioche) maoricum (Walker, 1869) and the ground crickets Bobilla nigrova (Swan, 1972) and B.bigelowi
We discuss the possibility of using calculating singularities to forecast the dynamics of hurricanes.Our basic model is the shallow-water system.By treating the hurricane eye as a vortex type Collections singularity and Mini Breastplate truncating the corresponding sequence of Hugoniot type conditions, we carry out many numerical experiments.The co
Dzhangar has a special place among texts of the folklore corpus, as it is considered to be the basic epic text of the Oirat-speaking ethnoses, having both regional and local versions.The analysis of the lexical structure of the Kalmyk version of the epic and namely of the Buddhist lexis, reflecting images of their creators regarding the religious a